nsPEF dramatically affects peritoneal carcinomatosis tumors in a syngeneic orthotopic graft murine model - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2019

nsPEF dramatically affects peritoneal carcinomatosis tumors in a syngeneic orthotopic graft murine model

Abdelkader Taibi
  • Fonction : Auteur
Marie-Laure Perrin
  • Fonction : Auteur
Jérémie Albouys
  • Fonction : Auteur
Catherine Yardin
Sylvaine Durand-Fontanier
Sylvia M. Bardet

Résumé

New immunocompetent cancer models are still needed for the identification and preclinical validation of novel therapeutic targets in colorectal cancer. We have developed 4 models by syngeneic intravenous, subcutaneous, intraperitoneal or laparotomy graft of luciferase expressing CT-26 cells with or w/o 10 ns PEF exposure, a promising tool for tumor ablation. Anatomic and histologic analysis corroborated the existence of tumoral nodules, and multiphoton microscopy was used to describe peritoneal tumoral fibrosis.In vitro CT-26 cells were also analyzed for their sensitivity to nsPEF (survival, mitochondrial potential, calcium waves and permeabilization). We were able to show a drastic effect of nsPEF on in vivo tumor morphology and survival.
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Dates et versions

hal-03032230 , version 1 (30-11-2020)

Identifiants

  • HAL Id : hal-03032230 , version 1

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Abdelkader Taibi, Marie-Laure Perrin, Jérémie Albouys, Catherine Yardin, Sylvaine Durand-Fontanier, et al.. nsPEF dramatically affects peritoneal carcinomatosis tumors in a syngeneic orthotopic graft murine model. BIOEM 2019, 2019, MONTPELLIER, France. ⟨hal-03032230⟩

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