nsPEF dramatically affects peritoneal carcinomatosis tumors in a syngeneic orthotopic graft murine model
Résumé
New immunocompetent cancer models are still needed for the identification and preclinical validation of novel therapeutic targets in colorectal cancer. We have developed 4 models by syngeneic intravenous, subcutaneous, intraperitoneal or laparotomy graft of luciferase expressing CT-26 cells with or w/o 10 ns PEF exposure, a promising tool for tumor ablation. Anatomic and histologic analysis corroborated the existence of tumoral nodules, and multiphoton microscopy was used to describe peritoneal tumoral fibrosis.In vitro CT-26 cells were also analyzed for their sensitivity to nsPEF (survival, mitochondrial potential, calcium waves and permeabilization). We were able to show a drastic effect of nsPEF on in vivo tumor morphology and survival.
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