Binding of a Soluble meso-Tetraarylporphyrin to Human Galectin-7 Induces Oligomerization and Modulates Its Pro-Apoptotic Activity - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemistry Année : 2020

Binding of a Soluble meso-Tetraarylporphyrin to Human Galectin-7 Induces Oligomerization and Modulates Its Pro-Apoptotic Activity

Yossef López De Los Santos
  • Fonction : Auteur
David N. Bernard
  • Fonction : Auteur
Philippe Egesborg
  • Fonction : Auteur
Myriam Létourneau
  • Fonction : Auteur
Clara Lafortune
  • Fonction : Auteur
Matthew J. Cuneo
  • Fonction : Auteur
David Chatenet
  • Fonction : Auteur
Jean-Pierre Mahy
  • Fonction : Auteur
Yves St-Pierre
  • Fonction : Auteur
Rémy Ricoux
Nicolas Doucet
  • Fonction : Auteur
  • PersonId : 759189
  • IdRef : 091563488

Résumé

The selective targeting of protein-protein interactions remains a significant determinant for the proper modulation and regulation of cell apoptosis. Prototypic galectins such as human galectin-7 (GAL-7) are characterized by their ability to form homodimers that control the molecular fate of a cell by mediating subtle yet critical glycan-dependent interactions between pro- and anti-apoptotic molecular partners. Altering the structural architecture of GAL-7 can therefore result in resistance to apoptosis in various human cancer cells, further illustrating its importance in cell survival. In this study, we used a combination of biophysical and cellular assays to illustrate that binding of a water-soluble meso-tetraarylporphyrin molecule to GAL-7 induces protein oligomerization and modulation of GAL-7-induced apoptosis in human Jurkat T cells. Our results suggest that the integrity of the GAL-7 homodimer architecture is essential for its molecular function, in addition to providing an interesting porphyrin binding modulator for controlling apoptosis in mammalian cells.

Dates et versions

hal-03031721 , version 1 (30-11-2020)

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Citer

Yossef López De Los Santos, David N. Bernard, Philippe Egesborg, Myriam Létourneau, Clara Lafortune, et al.. Binding of a Soluble meso-Tetraarylporphyrin to Human Galectin-7 Induces Oligomerization and Modulates Its Pro-Apoptotic Activity. Biochemistry, 2020, ⟨10.1021/acs.biochem.0c00736⟩. ⟨hal-03031721⟩
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