Distribution and genotoxic effects after successive exposure to different uranium oxide particles inhaled by rats
Résumé
In nuclear fuel cycle facilities, workers may inhale airborne uranium compounds that lead to internal contamination, with various exposure scenarios depending on the workplace. These exposures can be chronic, repeated, or acute, and can involve many different compounds. The effect of uranium after multiple scenarios of exposure is unknown. The aim of this study, therefore, was to investigate the genotoxic and biokinetics consequences of exposure to depleted insoluble uranium dioxide (UO 2 ) by repeated or acute inhalation on subsequent acute inhalation of moderately soluble uranium peroxide (UO 4 ) in rats. The results show that UO 2 repeated preexposure by inhalation increases the genotoxic effects of UO 4 inhalation, assessed by comet assay, in different cell types, when UO 4 exposure alone has no effect. At the same time, the study of UO 4 bioaccumulation showed that the UO 4 biokinetics in the kidneys, gastrointestinal tract, and excreta, but not in the lungs, were slightly modified by previous UO 2 exposures. All these results show that both genotoxic and biokinetics effects of uranium may depend on preexposure and that repeated exposure induces a potentiation effect compared with acute exposure. Copyright © Informa Healthcare.
Mots clés
Drug Synergism
Eating
Inhalation Exposure
Kidney
Male
Mutagens
Rats
Sprague-Dawley
Specific Pathogen-Free Organisms
Tissue Distribution
Uranium Compounds
unclassified drug
uranium derivative
uranium dioxide
uranium peroxide
mutagenic agent
animal cell
animal experiment
article
bioaccumulation
comet assay
controlled study
exposure
gastrointestinal tract
genotoxicity
inhalation
kidney
long term exposure
lung
male
nonhuman
priority journal
rat
aerosol
air pollutant
animal
body weight
classification
dose response
drinking
drug effect
drug potentiation
eating
germfree animal
pathology
Sprague Dawley rat
tissue distribution
Aerosols
Air Pollutants
Occupational
Animals
Body Weight
Comet Assay
Dose-Response Relationship
Drug
Drinking