To optimize the performance of fuel cells and to deepen the knowledge of their behavior, it is necessary to make measurements in real time. To run such a measure, it is necessary to use specific software and hardware tools to instrumentation fuel cells. In this context, we presented the steps of designing and developing an application for the measurement of the complex impedance by electrochemical impedance spectroscopy method (EIS) using LabVIEW® software. The experimental results are obtained using a Ballard Nexa® PEM fuel cell, an electronic load produced in our laboratory and application developed by the LabVIEW® software.