Chemical synthesis of native S‐palmitoylated membrane proteins through removable‐backbone‐modification‐assisted Ser/Thr ligation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Angewandte Chemie International Edition Année : 2019

Chemical synthesis of native S‐palmitoylated membrane proteins through removable‐backbone‐modification‐assisted Ser/Thr ligation

Résumé

Preparation of native S-palmitoylated (S-palm) membrane proteins represents one of the unsolved challenges in chemical protein synthesis. Herein, we reported the first chemical synthesis of S-palm membrane proteins by reversible backbone modification assisted Ser/Thr Ligation (RBM$^{GABA}$ -assisted STL). This method involves two critical steps: (1) Synthesis of S-palm peptides by a new γ-aminobutyric acid group-based RBM (RBM$^{GABA}$) strategy, (2) Ligation of the S-palm RBM-modified peptides to give the desired S-palm product by STL. The utility of RBM$^{GABA}$ -assisted STL method was demonstrated by the synthesis of rabbit S-palm sarcolipin (SLN) and the S-palm matrix-2 (M2) ion channel. The synthesis of S-palm membrane proteins highlights the importance of developing non-NCL methods for chemical protein synthesis.
Fichier principal
Vignette du fichier
Huang et al., 2019.pdf (2.06 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03016456 , version 1 (20-11-2020)

Licence

Paternité

Identifiants

Citer

Dong‐liang Huang, Cédric Montigny, Yong Zheng, Veronica Beswick, Ying Li, et al.. Chemical synthesis of native S‐palmitoylated membrane proteins through removable‐backbone‐modification‐assisted Ser/Thr ligation. Angewandte Chemie International Edition, 2019, 59 (13), pp.5178-5184. ⟨10.1002/anie.201914836⟩. ⟨hal-03016456⟩
53 Consultations
324 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More