P-glycoprotein (ABCB1) is involved in vitamin K efflux - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Food Chemistry Année : 2020

P-glycoprotein (ABCB1) is involved in vitamin K efflux

Résumé

ABCB1 (P-glycoprotein/MDR1) is a multidrug efflux transporter that has previously been involved in cholesterol and vitamin D metabolism. Our aim was to explore whether ABCB1 is also involved in vitamin K efflux. Vitamin K apical efflux was significantly decreased in presence of ABCB1 inhibitor in Caco-2 cells (−20.4%; p < 0.05) and increased in Griptite cells overexpressing ABCB1 (+40.7%; p < 0.05). In vivo, the vitamin K postprandial response was higher in male Abcb1−/− mice after gavage compared to control animals (+115%; p < 0.05), but was unchanged in female mice. Finally, a vitamin K transintestinal efflux and a biliary vitamin K efflux were observed, but the specific involvement of ABCB1 could not be confirmed in these pathways. Overall, we showed for the first time that ABCB1 is involved in enterocyte vitamin K efflux in both cell and mouse models and regulates vitamin K absorption in mice.

Dates et versions

hal-03012693 , version 1 (18-11-2020)

Identifiants

Citer

Marielle Margier, Cedric Le May, Tiffany Antoine, Charlotte Halimi, Marion Nowicki, et al.. P-glycoprotein (ABCB1) is involved in vitamin K efflux. Food Chemistry, 2020, 343, pp.1-5. ⟨10.1016/j.foodchem.2020.128510⟩. ⟨hal-03012693⟩
51 Consultations
34 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More