Random Illumination Microscopy from Variance Images - Archive ouverte HAL
Communication Dans Un Congrès Année : 2021

Random Illumination Microscopy from Variance Images

Anne Sentenac
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Thomas Mangeat

Résumé

We propose a reconstruction algorithm called al-goRIM for super-resolution fluorescence microscopy, based on speckle illuminations and image variance matching. Super-resolution with a factor two or close can be achieved under realistic conditions in terms of number of images and signal to noise ratio. Here, our key result is an approximation of the statistical variance equation, leading to a drastic reduction of the computational complexity. Moreover, we demonstrate that the unmodulated out-of-focus light does not contribute to the data variance, and that the statistical component due to noise can be estimated and removed in an unsupervised way, which is a crucial contribution to the practical robustness of algoRIM.
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Dates et versions

hal-03010108 , version 1 (17-11-2020)

Identifiants

Citer

Simon Labouesse, Jérôme Idier, Anne Sentenac, Thomas Mangeat, Marc Allain. Random Illumination Microscopy from Variance Images. EUSIPCO 2020, Jan 2021, Amsterdam, Netherlands. pp.785-789, ⟨10.23919/Eusipco47968.2020.9287651⟩. ⟨hal-03010108⟩
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