The nucleotide sequence of the PRII gene related to DNA primase in Saccharomyces cerevisiae - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 1987

The nucleotide sequence of the PRII gene related to DNA primase in Saccharomyces cerevisiae

Rostyslav Makarenko
  • Fonction : Auteur
Claire Denis
  • Fonction : Auteur
  • PersonId : 785151
  • IdRef : 19545233X
Serge Gangloff
  • Fonction : Auteur
Benoît Arcangioli

Résumé

The PRI1 gene of Saccharomyces cerevisiae encodes for the p48 polypeptide of DNA primase. We have determined the nucleotide sequence of a 1,965 bp DNA fragment containing the PRI1 locus. The entire coding sequence of the gene lies within an open reading frame, and there are 409 amino acids in the single polypeptide protein if translation is assumed to start at the first ATG in this frame. The 5' and 3' end-points of PRI1 mRNA have been determined by S1 mapping and primer extension analysis. The primary structure and the codon usage of PRI1 suggest that this essential gene is poorly expressed in yeast cells.

Dates et versions

hal-03007979 , version 1 (16-11-2020)

Identifiants

Citer

Rostyslav Makarenko, Claire Denis, Stefania Francesconi, Serge Gangloff, Benoît Arcangioli. The nucleotide sequence of the PRII gene related to DNA primase in Saccharomyces cerevisiae. Nucleic Acids Research, 1987, 15 (19), pp.7975-7989. ⟨10.1093/nar/15.19.7975⟩. ⟨hal-03007979⟩
9 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More