Article Dans Une Revue EMBO Reports Année : 2018

Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment

Isabelle Quéguiner
  • Fonction : Auteur
Agnieszka Kolano
  • Fonction : Auteur
Thomas Boudier
Philippe Mailly
  • Fonction : Auteur

Résumé

Mitotic spindles assemble from two centrosomes, which are major microtubule-organizing centers (MTOCs) that contain centrioles. Meiotic spindles in oocytes, however, lack centrioles. In mouse oocytes, spindle microtubules are nucleated from multiple acentri-olar MTOCs that are sorted and clustered prior to completion of spindle assembly in an "inside-out" mechanism, ending with establishment of the poles. We used HSET (kinesin-14) as a tool to shift meiotic spindle assembly toward a mitotic "outside-in" mode and analyzed the consequences on the fidelity of the division. We show that HSET levels must be tightly gated in meiosis I and that even slight overexpression of HSET forces spindle morphogenesis to become more mitotic-like: rapid spindle bipolarization and pole assembly coupled with focused poles. The unusual length of meio-sis I is not sufficient to correct these early spindle morphogenesis defects, resulting in severe chromosome alignment abnormalities. Thus, the unique "inside-out" mechanism of meiotic spindle assembly is essential to prevent chromosomal misalignment and production of aneuploidy gametes.

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hal-03003475 , version 1 (13-11-2020)

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Isma Bennabi, Isabelle Quéguiner, Agnieszka Kolano, Thomas Boudier, Philippe Mailly, et al.. Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment. EMBO Reports, 2018, 19, pp.368 - 381. ⟨10.15252/embr.201745225⟩. ⟨hal-03003475⟩
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