Genome editing by CRISPR-Cas9 technology in Petunia hybrida - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta Horticulturae Année : 2020

Genome editing by CRISPR-Cas9 technology in Petunia hybrida

Résumé

CRISPR-Cas9 technology has been shown to be a very powerful tool to generate targeted mutations in a wide range of species. To evaluate its applicability for standard gene functional analysis in Petunia, we performed a case study aimed at obtaining loss-of-function alleles for six different Petunia genes. Using single guide RNAs (sgRNA), we obtained between 1 to 3 putative null alleles for 5 out of the six genes. Here we describe in detail the obtained results and the protocol used in this study, and discuss some of the technical problems encountered. Although the efficiency of the protocol should be further increased, these results demonstrate that CRISPR-Cas9 technology represents a very useful addition to the available molecular toolkit in petunia.
Fichier principal
Vignette du fichier
Chopy et al -Genome editing by CRISPR-Cas9 technology in Petunia hybrida.pdf (888 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02999116 , version 1 (10-11-2020)

Identifiants

Citer

M. Chopy, Pierre Morel, S. Rodrigues Bento, M. Vandenbussche. Genome editing by CRISPR-Cas9 technology in Petunia hybrida. Acta Horticulturae, 2020, 1283, pp.209-218. ⟨10.17660/ActaHortic.2020.1283.28⟩. ⟨hal-02999116⟩
85 Consultations
262 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More