Article Dans Une Revue Journal of visualized experiments : JoVE Année : 2020

Traction Force Microscopy to Study B Lymphocyte Activation

Paolo Pierobon
Anita Kumari
  • Fonction : Auteur
Judith Pineau
  • Fonction : Auteur
Ana-Maria Lennon-Duménil
  • Fonction : Auteur
Martial M Balland
  • Fonction : Auteur

Résumé

Traction force microscopy (TFM) enables the measurement of forces produced by a cell on a substrate. This technique infers traction force measurements from an experimentally observed displacement field produced by a cell pulling on an elastic substrate. Here, we adapted TFM to investigate the spatial and temporal structure of the force field exerted by B cells when activated by antigen engagement of the B cell receptor. Gel rigidity, bead density, and protein functionalization must be optimized for the study of relatively small cells (~ 6 µm) that interact with, and respond specifically to ligands for cell surface receptors.

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Dates et versions

hal-02996888 , version 1 (09-11-2020)

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Paolo Pierobon, Anita Kumari, Judith Pineau, Ana-Maria Lennon-Duménil, Martial M Balland. Traction Force Microscopy to Study B Lymphocyte Activation. Journal of visualized experiments : JoVE, 2020, ⟨10.3791/60947⟩. ⟨hal-02996888⟩
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