The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation
Résumé
Aspartimide formation often complicates the solid-phase synthesis of peptides. Much less discussed is the potential occurrence of this side reaction during the coupling of peptide segments using chemoselective peptide bond-forming reactions such as the native chemical ligation and extended methods. Here we describe how to manage this problem using bis(2-sulfenylethyl)amido (SEA)-mediated ligation.
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