Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genetics Année : 2002

Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae.

Résumé

In budding yeast, loss of topoisomerase III, encoded by the TOP3 gene, leads to a genomic instability phenotype that includes slow growth, hyper-sensitivity to genotoxic agents, mitotic hyper-recombination, increased chromosome missegregation, and meiotic failure. Slow growth and other defects of top3 mutants are suppressed by mutation of SGS1, which encodes the only RecQ helicase in S. cerevisiae. sgs1 is epistatic to top3, suggesting that the two proteins act in the same pathway. To identify other factors that function in the Sgs1-Top3 pathway, we undertook a genetic screen for non-sgs1 suppressors of top3 defects. We found that slow growth and DNA damage sensitivity of top3 mutants are suppressed by mutations in RAD51, RAD54, RAD55, and RAD57. In contrast, top3 mutants show extreme synergistic growth defects with mutations in RAD50, MRE11, XRS2, RDH54, and RAD1. We also analyzed recombination at the SUP4-o region, showing that in a rad51, rad54, rad55, or rad57 background top3Delta does not increase recombination to the same degree as in a wild-type strain. These results suggest that the presence of the Rad51 homologous recombination complex in a top3 background facilitates creation of detrimental intermediates by Sgs1. We present a model wherein Rad51 helps recruit Sgs1-Top3 to sites of replicative damage.
Fichier principal
Vignette du fichier
Shor-Genetics.pdf (524.94 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02990088 , version 1 (14-04-2023)

Identifiants

Citer

Erika Shor, Serge Gangloff, Marisa Wagner, Justin Weinstein, Gavrielle Price, et al.. Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae.. Genetics, 2002, 162 (2), pp.647-62. ⟨10.1093/genetics/162.2.527⟩. ⟨hal-02990088⟩

Collections

PASTEUR
15 Consultations
21 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More