The Arabidopsis N-alpha-acetyltransferase NAA60 locates to the plasma membrane and is vital for the high salt stress response - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue New Phytologist Année : 2020

The Arabidopsis N-alpha-acetyltransferase NAA60 locates to the plasma membrane and is vital for the high salt stress response

Dirk Schwarzer

Résumé

In humans and plants, N-terminal acetylation plays a central role in protein homeostasis, affects 80 % of proteins in the cytoplasm and is catalyzed by five ribosome-associated N-acetyltransferases (NatA-E). Humans also possess a Golgi-associated NatF (HsNAA60) that is essential for Golgi integrity. Remarkably, NAA60 is absent in fungi and was not identified in plants. Here we identify and characterize the first plasma membrane-anchored post-translationally acting N-acetyltransferase AtNAA60 in the reference plant Arabidopsis thaliana by the combined application of reverse genetics, global proteomics, live-cell imaging, microscale thermophoresis, CD-spectroscopy, nano differential scanning fluorometry, intrinsic tryptophan fluorescence and X-ray crystallography. We demonstrate that AtNAA60 like HsNAA60 is membrane-localized in vivo by an α-helical membrane anchor at its C-terminus, but in contrast to HsNAA60, AtNAA60 localizes to the plasma membrane. The AtNAA60 crystal structure provides insights into substrate-binding, the broad substrate specificity and the catalytic mechanism probed by structure-based mutagenesis. Characterization of the NAA60 loss-of-function mutants (naa60-1 and naa60-2) uncovers a plasma membrane-localized substrate of AtNAA60 and the importance of NAA60 during high salt stress. Our findings provide evidence for the plant-specific evolution of a plasma membrane-anchored N-acetyltransferase that is vital for adaptation to stress.
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Dates et versions

hal-02989484 , version 1 (10-11-2020)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Eric Linster, Dominik Layer, Willy V Bienvenut, Trinh V. Dinh, Felix A. Weyer, et al.. The Arabidopsis N-alpha-acetyltransferase NAA60 locates to the plasma membrane and is vital for the high salt stress response. New Phytologist, 2020, 228 (2), pp.554-669. ⟨10.1111/nph.16747⟩. ⟨hal-02989484⟩
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