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Article Dans Une Revue NeuroImage Année : 2020

Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening

Résumé

Astrocytes are a major type of glial cell in the mammalian brain, essentially regulating neuronal development and function. Quantitative imaging represents an important approach to study astrocytic signaling in neural circuits. Focusing on astrocytic Ca 2þ activity, a key pathway implicated in astrocye-neuron interaction, we here report a strategy combining fast light sheet fluorescence microscopy (LSFM) and correlative screening-based time series analysis, to map activity domains in astrocytes in living mammalian nerve tissue. Light sheet of micron-scale thickness enables wide-field optical sectioning to image astrocytes in acute mouse brain slices. Using both chemical and genetically encoded Ca 2þ indicators, we demonstrate the complementary advantages of LSFM in mapping Ca 2þ domains in astrocyte populations as compared to epifluorescence and two-photon microscopy. Our approach then revealed distinct kinetics of Ca 2þ signals between cortical and hypothalamic astrocytes in resting conditions and following the activation of adrenergic G protein coupled receptor (GPCR). This observation highlights the activity heterogeneity across regionally distinct astrocyte populations, and indicates the potential of our method for investigating dynamic signals in astrocytes.

Domaines

Neurobiologie
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Dates et versions

hal-02989010 , version 1 (09-11-2020)

Identifiants

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Cuong Pham, Daniela Herrera Moro, Christine Mouffle, Steve Didienne, Régine Hepp, et al.. Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening. NeuroImage, 2020, 220, pp.117069. ⟨10.1016/j.neuroimage.2020.117069⟩. ⟨hal-02989010⟩
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