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Article Dans Une Revue International Journal of Molecular Sciences Année : 2020

The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes

Maxime Ducret
Jean-Christophe Farges
  • Fonction : Auteur
Marielle Pasdeloup
  • Fonction : Auteur
Andreas Mueller
  • Fonction : Auteur
Frédéric Mallein-Gerin
Hugo Fabre
  • Fonction : Auteur
Anne-Laure Durand
  • Fonction : Auteur
Alexandre Dufour
  • Fonction : Auteur
Elisabeth Aubert-Foucher
  • Fonction : Auteur
Christine Oger-Desfeux
  • Fonction : Auteur
Sebastien Lustig
Elvire Servien
Gualter Vaz
  • Fonction : Auteur
Jerome Lafont
  • Fonction : Auteur

Résumé

Osteoarthritis (OA) is a degenerative disease of the joints which is associated with an impaired production of the cartilage matrix by the chondrocytes. Here, we investigated the role of Lysine-Specific Demethylase-1 (LSD1), a chromatin remodeling enzyme whose role in articular chondrocytes was previously associated with a catabolic activity and which is potentially involved during OA. Following a loss of function strategy and RNA sequencing analysis, we detail the genes which are targeted by LSD1 in human articular chondrocytes and identify COL9A1, a gene encoding the ?1 chain of the cartilage-specific type IX collagen, as negatively regulated by LSD1. We show that LSD1 interacts with the transcription factor SOX9 and is recruited to the promoter of COL9A1. Interestingly, we observe that OA cartilage displays stronger LSD1 immunostaining compared with normal, and we demonstrate that the depletion of LSD1 in OA chondrocytes prevents the decrease in COL9A1 following Il-1? treatment. These results suggest LSD1 is a new regulator of the anabolic activity of articular chondrocytes potentially destabilizing the cartilage matrix, since it negatively regulates COL9A1, a gene encoding a crucial anchoring collagen molecule. This newly identified role played by LSD1 may thus participate in the alteration of the cartilage matrix during OA.
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Dates et versions

hal-02988372 , version 1 (09-06-2021)

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Emeline Perrier-Groult, Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Andreas Mueller, et al.. The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes. International Journal of Molecular Sciences, 2020, 21 (17), 16p. ⟨10.3390/ijms21176322⟩. ⟨hal-02988372⟩
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