The bacterial cell division protein fragment EFtsN binds to and activates the major peptidoglycan synthase PBP1b - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2020

The bacterial cell division protein fragment EFtsN binds to and activates the major peptidoglycan synthase PBP1b

Adrien Boes
  • Fonction : Auteur
Frédéric Kerff
  • Fonction : Auteur
Raphael Herman
  • Fonction : Auteur
Eefjan Breukink
  • Fonction : Auteur
Mohammed Terrak
  • Fonction : Auteur

Résumé

Peptidoglycan (PG) is an essential constituent of the bacterial cell wall. During cell division, the machinery responsible for PG synthesis localizes mid-cell, at the septum, under the control of a multiprotein complex called the divisome. In Escherichia coli, septal PG synthesis and cell constriction rely on the accumulation of FtsN at the division site. Interestingly, a short sequence of FtsN (L75 to Q93, known as EFtsN) was shown to be essential and sufficient for its functioning in vivo, but what exactly this sequence is doing remained unknown. Here, we show that EFtsN binds specifically to the major PG synthase PBP1b and is sufficient to stimulate its biosynthetic glycosyltransferase (GTase) activity. We also report the crystal structure of PBP1b in complex with EFtsN, which demonstrates EFtsN binds at the junction between the GTase and UB2H domains of PBP1b. Interestingly, mutations to two residues (R141A/R397A) within the EFtsN binding pocket reduced the activation of PBP1b by FtsN but not by the lipoprotein LpoB. This mutant was unable to rescue ΔponB-ponAts strain, that lack PBP1b and has a thermosensitive PBP1a, at nonpermissive temperature and induced a mild cell chaining phenotype and cell lysis. Altogether, the results show that EFtsN interacts with PBP1b and that this interaction plays a role in the activation of its GTase activity by FtsN, which may contribute to the overall septal PG synthesis and regulation during cell division.

Dates et versions

hal-02985460 , version 1 (02-11-2020)

Identifiants

Citer

Adrien Boes, Frédéric Kerff, Raphael Herman, Thierry Touzé, Eefjan Breukink, et al.. The bacterial cell division protein fragment EFtsN binds to and activates the major peptidoglycan synthase PBP1b. Journal of Biological Chemistry, 2020, ⟨10.1074/jbc.RA120.015951⟩. ⟨hal-02985460⟩
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