Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2020

Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation

Valentina Curcio
  • Fonction : Auteur
Sophie Brasselet
Miguel Alonso

Résumé

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging. Orientation is intrinsically coupled to position in microscopy imaging, and molecular wobbling during the image integration time can bias orientation measurements. Providing 3D molecular orientation and orientational fluctuations would offer new ways to assess the degree of alignment of protein structures, which cannot be monitored by pure localization. Here we demonstrate that by adding polarization control to phase control in the Fourier plane of the imaging path, all parameters can be determined unambiguously from single molecules: 3D spatial position, 3D orientation and wobbling or dithering angle. The method, applied to fluorescent labels attached to single actin filaments, provides precisions within tens of nanometers in position and few degrees in orientation.
Fichier principal
Vignette du fichier
s41467-020-19064-6.pdf (2.81 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02976818 , version 1 (23-10-2020)

Identifiants

Citer

Valentina Curcio, Luis A Alemán-Castañeda, Thomas G Brown, Sophie Brasselet, Miguel Alonso. Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation. Nature Communications, 2020, 11 (1), pp.5307. ⟨10.1038/s41467-020-19064-6⟩. ⟨hal-02976818⟩
94 Consultations
54 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More