Influence of dose and route of administration on the outcome of infection with the virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at mid-gestation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Veterinary Research Année : 2018

Influence of dose and route of administration on the outcome of infection with the virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at mid-gestation

Roberto Sánchez-Sánchez
  • Fonction : Auteur
Ignacio Ferre
  • Fonction : Auteur
Michela Re
  • Fonction : Auteur
Javier Regidor-Cerrillo
  • Fonction : Auteur
Javier Blanco-Murcia
  • Fonction : Auteur
Luis Miguel Ferrer
  • Fonction : Auteur
Teresa Navarro
  • Fonction : Auteur
Manuel Pizarro Díaz
  • Fonction : Auteur
Marta González-Huecas
  • Fonction : Auteur
Enrique Tabanera
  • Fonction : Auteur
Julio Benavides
  • Fonction : Auteur
Luis Miguel Ortega-Mora
  • Fonction : Auteur

Résumé

AbstractExperimental infections in pregnant sheep have been focused on studying the effect of the time of challenge on the outcome of N. caninum infection, whereas the impact of the dose and route of challenge has not been studied in depth. Therefore, clinical outcome, immune responses, parasite detection and burden, and lesion severity in placental tissues and foetal brains were investigated in 90-day-pregnant sheep inoculated intravenously with 105 (G1), 104 (G2), 103 (G3), or 102 (G4) tachyzoites or subcutaneously with 104 (G5) tachyzoites of the virulent Nc-Spain7 isolate and an uninfected group (G6). Comparing challenge doses, G1 was the only group that had 100% abortion. Likewise, IFNγ levels in G1 increased earlier than those in other intravenously infected groups, and IgG levels on day 21 post-infection (pi) were higher in G1 than those in other intravenously infected groups. Concerning vertical transmission, G1 shows a higher parasite burden in the foetal brain than did G2 and G3. Comparing routes of administration, no differences in foetal survival rate or parasite load in the foetal brain were found. Although G2 had higher IFNγ levels than G5 on day 10 pi, no differences were found in humoral immune responses. Because the outcome after intravenous infection with 105 tachyzoites was similar to that observed after intravenous infection with 106 tachyzoites used in a previous work (100% abortion and vertical transmission), we conclude that it may be reasonable to use 105 tachyzoites administered by the intravenous route in further experiments when assessing drugs or vaccine candidates.
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Dates et versions

hal-02973489 , version 1 (21-10-2020)

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Roberto Sánchez-Sánchez, Ignacio Ferre, Michela Re, Javier Regidor-Cerrillo, Javier Blanco-Murcia, et al.. Influence of dose and route of administration on the outcome of infection with the virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at mid-gestation. Veterinary Research, 2018, 49 (1), pp.42. ⟨10.1186/s13567-018-0539-5⟩. ⟨hal-02973489⟩

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