Article Dans Une Revue Frontiers in Bioengineering and Biotechnology Année : 2020

Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating

Miryam Criado-Gonzalez
Muhammad Haseeb Iqbal
Alain Carvalho
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Marc Schmutz
Loïc Jierry
Pierre Schaaf
Fouzia Boulmedais

Résumé

In western countries, one patient on twenty will develop a nosocomial infection during his hospitalization at health care facilities. Classical antibiotics being less and less effective, this phenomenon is expanding year after year. Prevention of bacteria colonization of implantable medical devices constitutes a major medical and financial issue. In this study, we developed an antibacterial coating based on self-assembled Fmoc-tripeptide. Fmoc-FFpY peptides (F: phenylalanine; Y: tyrosine; p: PO 4 2−) are dephosphorylated enzymatically into Fmoc-FFY by action of alkaline phosphatase functionalized silica nanoparticles (NPs@AP), previously deposited on a surface. Fmoc-FFY peptides then self-assemble through π-π stacking interactions, hydrogen bonds and hydrophobic interactions adopting β-sheets secondary structures. The obtained hydrogel coatings show fibrillary structures observed by cryo-scanning electron microscopy with a thickness of few micrometers. At low concentration (≤0.5 mg.mL −1), self-assembled Fmoc-FFY has a superior antibacterial activity than Fmoc-FFpY peptide in solution. After 24 h of incubation, Fmoc-FFY hydrogel coatings fully inhibit the development of Gram-positive Staphylococcus aureus (S. aureus). The antibacterial effect is maintained on an in vitro model of repetitive infection in the case of S. aureus. This coating could serve in infections were Gram positive bacteria are prevalent, e.g., intravascular catheter infections. This work gives new insights toward the design of an alternative antimicrobial coating.

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hal-02965385 , version 1 (13-10-2020)

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Miryam Criado-Gonzalez, Muhammad Haseeb Iqbal, Alain Carvalho, Marc Schmutz, Loïc Jierry, et al.. Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating. Frontiers in Bioengineering and Biotechnology, 2020, 8, ⟨10.3389/fbioe.2020.00938⟩. ⟨hal-02965385⟩
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