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Article Dans Une Revue International Journal of Biological Macromolecules Année : 2018

The pectinases from Sphenophorus levis: Potential for biotechnological applications

Danilo Elton Evangelista
  • Fonction : Auteur
Priscila Vasques Castilho
  • Fonction : Auteur
Flávio Henrique-Silva
  • Fonction : Auteur

Résumé

Pectinases represent about one fifth of the enzyme worldwide market due their wide range of biotechnological applications. Current commercial pectinases are exclusively obtained from microbial sources, but here we report a pectin methylesterase (Sl-PME) and an endo-polygalacturonase (Sl-EPG) bioprospected from the sugarcane weevil, Sphenophorus levis, which revealed good potential for industrial applications. Sl-PME and Sl-EPG were overexpressed in Pichia pastoris, purified and enzymatically characterized. Sl-EPG presents optimal activity at pH 4–5 and 50 °C, showing that it can be used for juice extraction and clarification. On the other hand, Sl-PME presents optimal activity at pH 6–8 and 40 °C, and thus, suitable for both acidic and alkaline processing, such as coffee and tea fermentation. Sl-EPG shows Vmax = 3.23 mM/min, KM = 2.4 g/L and kcat = 418.6 s−1. While Sl-PME shows Vmax = 0.14 mM/min, KM = 4.1 g/L and kcat = 1.7 s−1. A PG inhibitor (PGIP2) weakly interfered in the Sl-EPG activity and Sl-PME was not affected by a usual PME inhibitor. Moreover, these enzymes manifested synergistic action towards methylesterified pectin. Here, we propose these enzymes as novel alternative tools for the current commercial pectinases.
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Dates et versions

hal-02948253 , version 1 (24-09-2020)

Identifiants

Citer

Olivier Habrylo, Danilo Elton Evangelista, Priscila Vasques Castilho, Jérôme Pelloux, Flávio Henrique-Silva. The pectinases from Sphenophorus levis: Potential for biotechnological applications. International Journal of Biological Macromolecules, 2018, 112, pp.499-508. ⟨10.1016/j.ijbiomac.2018.01.172⟩. ⟨hal-02948253⟩
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