New Linear Lipopeptides Produced by Pseudomonas cichorii SF1-54 Are Involved in Virulence, Swarming Motility, and Biofilm Formation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Plant-Microbe Interactions Année : 2013

New Linear Lipopeptides Produced by Pseudomonas cichorii SF1-54 Are Involved in Virulence, Swarming Motility, and Biofilm Formation

Chien-Jui Huang
  • Fonction : Auteur
Marc Ongena
Valérie Leclère
Philippe Jacques
Peter Bleyaert
  • Fonction : Auteur
Herbert Budzikiewicz
  • Fonction : Auteur
Mathias Schäfer
  • Fonction : Auteur
Monica Höfte

Résumé

Pseudomonas cichorii is the causal agent of lettuce midrib rot, characterized by a dark-brown to green-black discoloration of the midrib. Formation of necrotic lesions by several plant-pathogenic pseudomonads is associated with production of phytotoxic lipopeptides, which contribute to virulence. Therefore, the ability of P. cichorii SF1-54 to produce lipopeptides was investigated. A cell-free culture filtrate of SF1-54 showed surfactant, antimicrobial, and phytotoxic activities which are typical for lipopeptides. High-performance liquid chromatography analysis of P. cichorii SF1-54 culture filtrate revealed the presence of seven compounds with lipopeptide characteristics. Two related lipopeptides, named cichofactin A and B, were studied in more detail: they are linear lipopeptides with a decanoic and dodecanoic lipid chain, respectively, connected to the N-terminus of an eight-amino-acid peptide moiety. Both cichofactins are new members of the syringafactin lipopeptide family. Furthermore, two nonribosomal peptide synthethase-encoding genes, cifA and cifB, were identified as responsible for cichofactin biosynthesis. A cifAB deletion mutant no longer produced cichofactins and was impaired in swarming motility but showed enhanced biofilm formation. Upon spray inoculation on lettuce, the cichofactin-deficient mutant caused significantly less rotten midribs than the wild type, indicating that cichofactins are involved in pathogenicity of P. cichorii SF1-54. Further analysis revealed that P. cichorii isolates vary greatly in swarming motility and cichofactin production.

Dates et versions

hal-02945832 , version 1 (22-09-2020)

Identifiants

Citer

Ellen Pauwelyn, Chien-Jui Huang, Marc Ongena, Valérie Leclère, Philippe Jacques, et al.. New Linear Lipopeptides Produced by Pseudomonas cichorii SF1-54 Are Involved in Virulence, Swarming Motility, and Biofilm Formation. Molecular Plant-Microbe Interactions, 2013, 26 (5), pp.585-598. ⟨10.1094/MPMI-11-12-0258-R⟩. ⟨hal-02945832⟩

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