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Article Dans Une Revue Journal of Infection in Developing Countries Année : 2020

Spoligotyping of Mycobacterium tuberculosis isolates using Luminex®-based method in Lebanon

Khaldoun Masoud
  • Fonction : Auteur
George F. Araj
  • Fonction : Auteur
Lina Reslan
  • Fonction : Auteur
  • PersonId : 763491
  • IdRef : 171654722
Sukayna Fadlallah
  • Fonction : Auteur
Michel Wehbe
  • Fonction : Auteur
Lina Itani
  • Fonction : Auteur
Aline Avedissian
  • Fonction : Auteur
Ghassan Dbaibo
  • Fonction : Auteur
Antoine Saade
  • Fonction : Auteur
Ghassan M. Matar
  • Fonction : Auteur

Résumé

INTRODUCTION: Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in Lebanon are scarce. This study was undertaken to reveal the spoligotypes of MTB isolates recovered from patients in Lebanon. METHODOLOGY: MTB isolates from 49 patients living in Lebanon were recovered and identified. The samples were heat killed and subjected to DNA extraction. Spoligotyping was performed using microbeads from TB-SPOL Kit and the fluorescence intensity was measured using Luminex 200®. Generated patterns were assigned to families using the SITVIT2 international database of the Pasteur Institute of Guadeloupe and compared. RESULTS: The spoligotyping of the 49 MTB isolates revealed that 31 isolates belonged to Lineage 4 (Euro-American, 63.3%), 12 to Lineage 3 (East- African Indian, 24.5%), 3 to Lineage 2 (East Asian, 6%) and 2 were unknown. Over half of the genotypes (16 of 30) harbored SIT127 supposed to belong to the L4.5 sublineage. One isolate belonging to the rare Manu-Ancestor SIT523 was recovered for the first time in Lebanon, being associated with highly virulent extensively drug-resistant (XDR) MTB phenotype. CONCLUSION: The application of the Spoligotyping Multiplex Luminex® method is an efficient, discriminatory and rapid method to use for first-lane genotyping of MTB isolates. Though humble numbers were tested, this study is one of the first to describe the genomic diversity and epidemiology of MTB isolates of Lebanon, and suggests an increasing prevalence of SIT127 in the country.

Dates et versions

hal-02944245 , version 1 (21-09-2020)

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Citer

Khaldoun Masoud, George F. Araj, Lina Reslan, Sukayna Fadlallah, Michel Wehbe, et al.. Spoligotyping of Mycobacterium tuberculosis isolates using Luminex®-based method in Lebanon. Journal of Infection in Developing Countries, 2020, 14 (8), pp.878--885. ⟨10.3855/jidc.12072⟩. ⟨hal-02944245⟩
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