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Article Dans Une Revue Talanta Open Année : 2020

4-Methoxy-ortho -phthalaldehyde: a promising derivatizing agent for the fluorimetric evaluation of histamine in seafood

Résumé

Histamine, the molecule associated with food poisoning, is nowadays considered as a reliable marker of seafood freshness and therefore its rapid and easy quantification appears critical. Existing method like HPLC or direct fluorimetric analysis employing ortho-phthalaldehyde (OPA) can be time-consuming and tedious. To overcome this problem, we propose in this paper to evaluate the application potential of 4-methoxy-ortho-phthalaldehyde (MO-OPA) for the rapid and easy evaluation of histamine in seafood samples. For this purpose, the histamine com-plexation conditions were studied by molecular fluorescence spectroscopy using MO-OPA as derivatization agent and then compared with the reference compounds: OPA. MOPA proved to be superior to OPA for histamine detection in terms of linearity range (1.10 − 6-1.10 − 4 mol.L − 1 , R 2 > 0.99, n = 10) and detection limit (9.5.10 − 7 mol.L − 1). The optimal conditions were next applied to the analysis of histamine in a mixture of biogenic amines standards and finally on real samples of yellowfin tuna of known histamine concentration.
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Dates et versions

hal-02942358 , version 1 (17-09-2020)

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Clémence Moitessier, Khémesse Kital, Pierre-Edouard Danjou, Francine Cazier-Dennin. 4-Methoxy-ortho -phthalaldehyde: a promising derivatizing agent for the fluorimetric evaluation of histamine in seafood. Talanta Open, 2020, 2, pp.100014. ⟨10.1016/j.talo.2020.100014⟩. ⟨hal-02942358⟩
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