Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue ACS Applied Materials & Interfaces Année : 2020

Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection

Résumé

Immobilization of biosensors in or on a functional material is critical for subsequent device development and translation to wearable technology. Here we present the development and assessment of an immobilized quantum dot - transcription factor - nucleic acid complex for progesterone detection as a first step toward such device integration. The sensor is composed of a polyhistidine-tagged transcription factor linked to a quantum dot and a fluorophore-modified cognate DNA, and embedded within a hydrogel as an immobilization matrix. The hydrogel is optically transparent, soft, and flexible as well as traps the quantum dot - transcription factor DNA assembly but allows free passage of the analyte, progesterone. Upon progesterone exposure, DNA dissociates from the quantum dot - transcription factor DNA assembly resulting in an attenuated ratiometric fluorescent output via Förster resonance energy transfer. The sensor performs in a dose-dependent manner with a limit of detection of 55 nM. Repeat analyte measurements are also similarly successful. Our approach combines a systematically characterized hydrogel as an immobilization matrix and a transcription factor - DNA binding as a recognition/ transduction element, offering a promising framework for future biosensor devices based upon allosteric transcription factor.
Fichier principal
Vignette du fichier
Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection_HAL.pdf (885.48 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02939691 , version 1 (15-09-2020)

Identifiants

Citer

Mingfu Chen, Chloé Grazon, Prerana Sensharma, Thuy Nguyen, Yunpeng Feng, et al.. Hydrogel embedded quantum dot-transcription factor sensor for quantitative progesterone detection. ACS Applied Materials & Interfaces, 2020, 12 (39), pp.43513-43521. ⟨10.1021/acsami.0c13489⟩. ⟨hal-02939691⟩

Collections

CNRS INC-CNRS LCPO
95 Consultations
323 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More