Microfluidic device to study flow-free chemotaxis of swimming cells - Archive ouverte HAL
Journal Articles Lab on a Chip Year : 2020

Microfluidic device to study flow-free chemotaxis of swimming cells

Abstract

Microfluidic devices have been used in the last two decades to study in vitro cell chemotaxis, but few existing devices generate gradients in flow-free conditions. Flow can bias cell directionality of adherent cells and precludes the study of swimming cells like naïve T lymphocytes, which only migrate in a non-adherent fashion. We developed two devices that create stable, flow-free, diffusion-based gradients and are adapted for adherent and swimming cells. The flow-free environment is achieved by using agarose gel barriers between a central channel with cells and side channels with chemoattractants. These barriers insulate cells from injection/rinsing cycles of chemoattractants, they dampen residual drift across the device, and they allow co-culture of cells without physical interaction, to study contactless paracrine communication. Our devices were used here to investigate neutrophil and naïve T lymphocyte chemotaxis.
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Dates and versions

hal-02932429 , version 1 (07-09-2020)

Identifiers

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Nicolas Garcia-Seyda, Laurene Aoun, Victoria Tishkova, Valentine Seveau, Martine Biarnes-Pelicot, et al.. Microfluidic device to study flow-free chemotaxis of swimming cells. Lab on a Chip, 2020, 20 (9), pp.1639-1647. ⟨10.1039/d0lc00045k⟩. ⟨hal-02932429⟩
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