Development of twelve novel polymorphic microsatellite DNA markers for the Boeseman’s rainbowfish (Melanotaenia boesemani) and tests for their cross-utility in 21 rainbowfish species from West Papua (Indonesia)
Résumé
We developed 12 microsatellite markers for the
endangered Boeseman’s rainbowfish (Melanotaenia
boesemani). Twenty-eight individuals from the type locality
at Ayamaru Lake were examined, and all loci were polymorphic
with a number of alleles per locus varying from 3 to 18.
Average observed and expected heterozygosities were 0.681
and 0.678, respectively. Cross-species amplification was successfully
obtained for 21 Melanotaenia species, with a number
of alleles per locus ranging between 1 and 20. Average
observed and expected heterozygosities varied between 0.105
and 0.708 and 0.118–0.755, respectively. Only 21 inbreeding
coefficient (Fis) values presented a significant homozygote
excess among the 264 locus-by-locus calculated values. Tests
for genotyping errors revealed that four of these 21 significant
Fis values could be explained by the presence of null alleles.
These new microsatellite markers appear highly reliable for
further conservation purposes or population genetic studies of
the many rainbowfish endangered species.