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Article Dans Une Revue ICES Journal of Marine Science Année : 2020

State of art and best practices for fatty acid analysis in aquatic sciences

Loic Michel

Résumé

Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and inter laboratory calibration.
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Dates et versions

hal-02920465 , version 1 (17-12-2020)

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Lydie I.E. Couturier, Loic Michel, Teresa Amaro, Suzanne Budge, Elisabete da Costa, et al.. State of art and best practices for fatty acid analysis in aquatic sciences. ICES Journal of Marine Science, 2020, ⟨10.1093/icesjms/fsaa121⟩. ⟨hal-02920465⟩
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