Sequence of the 5'-flanking region and promoter activity of the human mucin gene MUC5B in different phenotypes of colon cancer cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2000

Sequence of the 5'-flanking region and promoter activity of the human mucin gene MUC5B in different phenotypes of colon cancer cells

Résumé

Control of gene expression in intestinal cells is poorly understood. Molecular mechanisms that regulate transcription of cellular genes are the foundation for understanding developmental and differentiation events. Mucin gene expression has been shown to be altered in many intestinal diseases and especially cancers of the gastrointestinal tract. Towards understanding the tran-scriptional regulation of a member of the 11p15.5 human mucin gene cluster, we have characterized 3.55 kb of the 5h-flanking region of the human mucin gene MUC5B, including the promoter , the first two exons and the first intron. We report here the promoter activity of successively 5h-truncated sections of 956 bases of this region by fusing it to the coding region of a luciferase reporter gene. The transcription start site was determined by primer-extension analysis. The region upstream of the transcription start site is characterized by the presence of a TATA box at bases k32\k26, DNA-binding elements for transcription factors c-Myc, N-Myc, Sp1 and nuclear factor κB as well as putative activator protein (AP)-1-, cAMP-response-element-binding protein (CREB)-, hepatocyte nuclear
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Dates et versions

hal-02905708 , version 1 (29-09-2020)

Identifiants

  • HAL Id : hal-02905708 , version 1

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Isabelle van Seuningen, Michaël Perrais, Pascal Pigny, Nicole Porchet, Jean-Pierre Aubert. Sequence of the 5'-flanking region and promoter activity of the human mucin gene MUC5B in different phenotypes of colon cancer cells. Biochemical Journal, 2000, 348, pp.675 - 686. ⟨hal-02905708⟩
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