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Poster De Conférence Année : 2017

Response of zebrafish and Japanese medaka to imidacloprid: a comparative study

Résumé

Neonicotinoids are among the most produced and used insecticides and are frequently found in freshwater with concentrations ranging from ng to μg/L. These molecules are small and very soluble in water. This given, they are likely to pass the chorion and thus easily enter embryos of fish. It is known that these compounds impact on insects, including bees, in terms of toxicity and behaviour, but whether similar impacts could be observed in fish is not yet known. The aim of this study was to investigate effects of exposure to imidacloprid, one of the most used neonicotinoids, on the development and behaviour of two common fish lab species: Zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes). Fish were exposed during 5 (zebrafish) and 13 days (medaka) to 0, 0.2, 2, 20, 200 and 2000 μg/L imidacloprid by aqueous exposure, matching the respective time from fertilization to emerged larvae for the two species. At similar developmental stages, survival, hatch, growth, morphology, behaviour and histology were examined. No impact on survival was found. A delay for 2 μg/L imidacloprid exposures was observed for hatch in both species. Except for the 20 mg/L exposure level, imidacloprid led to hypoactivity in both species. A striking difference was found in morphology: a high number of deformities were noticed for medaka whereas almost none were found in zebrafish. Yolk and bone oedemas started to be noticed at 20 μg/L after hatch, jaw deformity and lordosis/scoliosis increased with concentration level. An increase of haemorrhage was found at the highest concentration (2000 μg/L). In order to shed light on the observed species differences, we intend to investigate the uptake of imidacloprid into the fish embryos and its biotransformation.
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Dates et versions

hal-02900347 , version 1 (16-07-2020)

Identifiants

  • HAL Id : hal-02900347 , version 1

Citer

Caroline Vignet, Kévin Lajoie, Maria Maisano, Hélène Mottaz, Christelle Clérandeau, et al.. Response of zebrafish and Japanese medaka to imidacloprid: a comparative study. SETAC Europe 27th Annual Meeting, May 2017, Bruxelle, Belgium. Society of Environmental Toxicology and Chemistry Europe (SETAC Europe), pp.298, SETAC Europe 27th Annual Meeting - Environmental Quality Through Transdisciplinary Collaboration. ⟨hal-02900347⟩

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