The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics - Archive ouverte HAL Access content directly
Journal Articles Biochimica biophysica acta (BBA) - Bioenergetics Year : 2020

The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics

Abstract

The three presently known enzymes responsible for arsenic-using bioenergetic processes are arsenite oxidase (Aio), arsenate reductase (Arr) and alternative arsenite oxidase (Arx), all of which are molybdoenzymes from the vast group referred to as the Mo/W-bisPGD enzyme superfamily. Since arsenite is present in substantial amounts in hydrothermal environments, frequently considered as vestiges of primordial biochemistry, arsenite-based bioenergetics has long been predicted to be ancient. Conflicting scenarios, however, have been put forward proposing either Arr/Arx or Aio as operating in the ancestral metabolism. Phylogenetic data argue in favor of Aio whereas biochemical and physiological data led several authors to propose Arx/Arr as the most ancient anaerobic arsenite metabolizing enzymes. Here we combine phylogenetic approaches with physiological and biochemical experiments to demonstrate that the Arx/Arr enzymes could not have been functional in the Archaean geological eon. We propose that Arr reacts with menaquinones to reduce arsenate whereas Arx reacts with ubiquinone to oxidize arsenite, in line with thermodynamic considerations. The distribution of the quinone biosynthesis pathways, however, clearly indicates that the ubiquinone pathway is recent. An updated phylogeny of Arx furthermore reinforces the hypothesis of a recent emergence of this enzyme. We therefore conclude that anaerobic arsenite redox conversion in the Archaean must have been performed in a metabolism involving Aio.
Fichier principal
Vignette du fichier
BBABIO-20-03_R2_unmarked.pdf (979.98 Ko) Télécharger le fichier
AppendixB_BBABIO-20-3_R2_unmarked.pdf (855.06 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02896357 , version 1 (21-09-2020)

Identifiers

Cite

Julie Szyttenholm, Florence Chaspoul, Marielle Bauzan, Anne-Lise Ducluzeau, Mahmoud Hajj Chehade, et al.. The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics. Biochimica biophysica acta (BBA) - Bioenergetics, 2020, 1861 (10), pp.148252. ⟨10.1016/j.bbabio.2020.148252⟩. ⟨hal-02896357⟩
2344 View
337 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More