In southeastern France, many Final Acheulean/Early Middle Palaeolithic and Middle Palaeolithic assemblages have yielded bone retouchers. The oldest are dated to the Middle Pleistocene: from MIS 11 at Terra Amata; MIS 9 at Orgnac 3; and MIS 6-7 at Payre F, Sainte-Anne I and Le Lazaret. However, this early evidence of bone tool use only concerns a few dozen pieces among thousands of faunal and lithic remains. These re-touchers indicate behavioural changes from MIS 11-9 onwards in southeastern France, associated with a mosaic of technological and subsistence changes that became more common during the Middle Palaeolithic. The frequency of these bone artefacts increases during MIS 7, becoming much more numerous after MIS 5, sometimes totaling more than a hundred items at one site, such as Saint-Marcel Cave. Bone retoucher frequency is still highly variable throughout the Middle Palaeolithic and seems to be determined by the type of occupation and activities rather than the associated lithic technologies. This broad, regional comparative analysis contributes to a better understanding of the technical behaviour developed by Neanderthals, as well as their Middle Pleistocene ancestors, and their ability to recover and use bones.