A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae - Archive ouverte HAL Access content directly
Journal Articles NAR Genomics and Bioinformatics Year : 2020

A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae

Abstract

The order Nidovirales is a diverse group of (+)RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes , despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviri-dae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2-OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence , or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a puta-tive Rossmann fold RNA methyltransferase in 10 To-baniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.

Domains

Virology
Fichier principal
Vignette du fichier
lqz022.pdf (2.05 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

hal-02890583 , version 1 (07-07-2020)

Licence

Attribution - NonCommercial

Identifiers

Cite

Francois Ferron, Humberto Julio Debat, Ashleigh Shannon, Etienne Decroly, Bruno Canard. A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae. NAR Genomics and Bioinformatics, 2020, 2 (1), ⟨10.1093/nargab/lqz022⟩. ⟨hal-02890583⟩
37 View
31 Download

Altmetric

Share

Gmail Facebook X LinkedIn More