Determination of anti-adeno-associated virus vector neutralizing antibody titer with an in vitro reporter system - Archive ouverte HAL
Article Dans Une Revue Hum Gene Ther Methods Année : 2015

Determination of anti-adeno-associated virus vector neutralizing antibody titer with an in vitro reporter system

Résumé

Adeno-associated virus (AAV) vectors are a platform of choice for in vivo gene transfer applications. However, neutralizing antibodies (NAb) to AAV can be found in humans and some animal species as a result of exposure to the wild-type virus, and high-titer NAb develop following AAV vector administration. In some conditions, anti-AAV NAb can block transduction with AAV vectors even when present at low titers, thus requiring prescreening before vector administration. Here we describe an improved in vitro, cell-based assay for the determination of NAb titer in serum or plasma samples. The assay is easy to setup and sensitive and, depending on the purpose, can be validated to support clinical development of gene therapy products based on AAV vectors.

Dates et versions

hal-02880998 , version 1 (25-06-2020)

Identifiants

Citer

A. Meliani, C. Leborgne, S. Triffault, L. Jeanson-Leh, P. Veron, et al.. Determination of anti-adeno-associated virus vector neutralizing antibody titer with an in vitro reporter system. Hum Gene Ther Methods, 2015, 26 (2), pp.45-53. ⟨10.1089/hgtb.2015.037⟩. ⟨hal-02880998⟩
35 Consultations
0 Téléchargements

Altmetric

Partager

More