Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus - Archive ouverte HAL Access content directly
Journal Articles DNA Research Year : 2020

Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus

Hayat Hage
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  • PersonId : 1036180
Elodie Drula
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  • PersonId : 1017641
Sacha Grisel
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  • PersonId : 1203965
Steven Ahrendt
Kerrie Barry
Kurt Labutti
Emmanuelle Morin
Eric Record

Abstract

White-rot (WR) fungi are pivotal decomposers of dead organic matter in forest ecosystems and typically use a large array of hydrolytic and oxidative enzymes to deconstruct lignocellulose. However, the extent of lignin and cellulose degradation may vary between species and wood type. Here we combined comparative genomics, transcriptomics and secretome proteomics to identify conserved enzymatic signatures at the onset of wood decaying activity within the Basidiomycota genus Pycnoporus. We observed strong conservation in the genome structures and the repertoires of protein coding genes across the four Pycnoporus species described to date, despite the species having distinct geographic distributions. We further analyzed the early response of P. cinnabarinus, P. coccineus and P. sanguineus to diverse (ligno)-cellulosic substrates. We identified a conserved set of enzymes mobilized by the three species for breaking down cellulose, hemicellulose and pectin. The co-occurrence in the exo-proteomes of H2O2 producing enzymes with H2O2 consuming enzymes was a common feature of the three species, although each enzymatic partner displayed independent transcriptional regulation. Finally, cellobiose dehydrogenase-coding genes were systematically co-regulated with at least one AA9 LPMO gene, indicative of enzymatic synergy in vivo. This study highlights a conserved core white-rot fungal enzymatic mechanism behind the wood decaying process.
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hal-02868456 , version 1 (18-10-2023)
hal-02868456 , version 2 (05-01-2024)

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Shingo Miyauchi, Hayat Hage, Elodie Drula, Laurence Lesage-Meessen, Jean-Guy Berrin, et al.. Conserved white rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus. DNA Research, 2020, ⟨10.1093/dnares/dsaa011⟩. ⟨hal-02868456v2⟩
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