Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2015

Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples

Résumé

NMR spectroscopy allows measurements of very accurate values of equilibrium dissociation constants using chemical shift perturbation methods, provided that the concentrations of the binding partners are known with high precision and accuracy. The accuracy and precision of these experiments are improved if performed using individual capillary tubes, a method enabling full automation of the measurement. We provide here a protocol to set up and perform these experiments as well as a robust method to measure peptide concentrations using tryptophan as an internal standard.

Domaines

Autre
Fichier principal
Vignette du fichier
publis16-agap-004_koler_accurate_1.pdf (491.58 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02801062 , version 1 (05-06-2020)

Identifiants

Citer

Christian Kohler, Raphaël Recht, Marc Quinternet, Frédéric de Lamotte, Marc-André Delsuc, et al.. Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples. Affinity Chromatography : Methods and Protocols, 1286, Humana Press, 341 p., 2015, Methods in Molecular Biology, 978-1-4939-2447-9. ⟨10.1007/978-1-4939-2447-9_22⟩. ⟨hal-02801062⟩
91 Consultations
374 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More