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Communication Dans Un Congrès Année : 2013

Molecular epidemiology provides new insights on ZYMV occurence in France

Karine Berthier
Cecile Desbiez

Résumé

Zucchini yellow mosaic virus (ZYMV) causes locally severe but sporadic epidemics in France, contrasting with other non-persistent aphid-borne viruses such as Watermelon mosaic virus (WMV) or Cucumber mosaic virus (CMV) which occur much more regularly. In order to better understand parameters that could explain this particular behavior, ZYMV occurrence and spread were monitored over a four-year period in a multilocal layout. The layout included four identical zucchini squash plots, planted on the same day with the same batch of seedlings and located 0.5 to 4 km apart. ZYMV, WMV, CMV and Cucurbit aphid-borne yellows virus (CABYV) epidemics were monitored within each plot at weekly intervals using DAS-ELISA and ZYMV populations were characterized by direct sequencing of the N-terminal part of the coat protein after RT-PCR amplification. ZYMV occurred irregularly, and, depending on the plot, developed either early, late or no epidemics. Three out of the five different strain types known from France were observed in the layout throughout the four years, but generally a single very homogeneous population (often composed of a single haplotype) was observed per plot. Only in one occasion, two distinct populations were detected in the same plot, and with different haplotypes for each population. These results will be discussed in relation with the potential of ZYMV dissemination by aphids, the origin of primary inoculum and the potential interactions between different virus populations.
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Dates et versions

hal-02747957 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-02747957 , version 1
  • PRODINRA : 257674

Citer

Hervé Lecoq, Catherine Wipf-Scheibel, Karine Berthier, Cecile Desbiez. Molecular epidemiology provides new insights on ZYMV occurence in France. 12. IPVE Symposium, International Committee on Plant Virus Epidemiology (ISPP). St Paul.; International Institute of Tropical Agriculture, CGIAR (IITA). Ibadan, NGA., Jan 2013, Arusha, Tanzania. 256 p. ⟨hal-02747957⟩
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