Article Dans Une Revue Applied Microbiology and Biotechnology Année : 2000

Inhibition of Clostridium butyricum by 1,3-propanediol and diols during glycerol fermentation

Résumé

1,3-Propanediol inhibition during glycerol fermentation to 1,3-propanediol by Clostridium butyricum CNCM 1211 has been studied. The initial concentration of the 1,3-propanediol affected the growth of the bacterium more than the glycerol fermentation. μ max was inversely proportional to the initial concentration of 1,3-propanediol (0–65 g l−1). For glycerol at 20 g l−1, the growth and fermentation were completely stopped at an initial 1,3-propanediol concentration of 65 g l−1. However, for an initial 1,3-propanediol concentration of 50 g l−1 and glycerol at 70 g l−1, the final concentration (initial and produced) of 1,3-propanediol reached 83.7 g l−1(1.1 M), with complete consumption of the glycerol. Therefore, during the fermentation, the strain tolerated a 1,3-propanediol concentration higher than the initial inhibitory concentration (65 g l−1). The addition of 1,2-propanediol or 2,3-butanediol (50 g l−1) in the presence of glycerol (50–100 g l−1), showed that 2-diols reduced the μ max in a similar way to 1,3-propanediol. The measurement of the osmotic pressure of glycerol solutions, diols and diol/glycerol mixtures did not indicate any differences between these compounds. The hypothesis of diol inhibition was discussed. Taking into account the strain tolerance of highly concentrated 1,3-propanediol during fermentation, the fermentation processes for optimising production were considered.

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Dates et versions

hal-02698797 , version 1 (01-06-2020)

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  • HAL Id : hal-02698797 , version 1
  • PRODINRA : 65125

Citer

T. Colin, A. Bories, Gabrielle Moulin. Inhibition of Clostridium butyricum by 1,3-propanediol and diols during glycerol fermentation. Applied Microbiology and Biotechnology, 2000, 54 (2), pp.201-205. ⟨hal-02698797⟩

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