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Article Dans Une Revue Journal of General Virology Année : 2002

An ex vivo murine model to study poliovirus-induced apoptosis in nerve cells

Résumé

Paralytic poliomyelitis results from destruction of motor neurons owing to poliovirus (PV) replication. Using a mouse model, we have previously shown that PV kills neurons of the central nervous system (CNS) as a result of apoptosis (Girard et al., Journal of Virology 73, 6066-6072, 1999). We report the development of mixed mouse primary nerve cell cultures from the cerebral cortex of neonatal mice transgenic for the human PV receptor. These cultures contained all three main cell types of the CNS, i.e. neurons, astrocytes and oligodendrocytes. All three cell types were susceptible to PV infection and virus replication in the cultures led to DNA fragmentation characteristic of apoptosis. PV-induced apoptosis was inhibited by the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD.FMK), indicating that this process involved caspases. Thus, these mixed mouse primary nerve cell cultures are a new in vitro model for studying the molecular mechanisms of PV-induced apoptosis in nerve cells.

Dates et versions

hal-02683592 , version 1 (01-06-2020)

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Citer

Therese Couderc, Florence Guivel-Benhassine, Viviane Calaora, Anne-Sophie Gosselin, Bruno Blondel. An ex vivo murine model to study poliovirus-induced apoptosis in nerve cells. Journal of General Virology, 2002, 83 (8), pp.1925-1930. ⟨10.1099/0022-1317-83-8-1925⟩. ⟨hal-02683592⟩

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