Determination of lactic acid bacteria producing biogenic amines in wine by quantitative PCR methods - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Letters in Applied Microbiology Année : 2008

Determination of lactic acid bacteria producing biogenic amines in wine by quantitative PCR methods

Olivier Claisse
  • Fonction : Auteur
Gilles de Revel
  • Fonction : Auteur
Aline Lonvaud-Funel
  • Fonction : Auteur
Patrick Lucas

Résumé

Aims: To develop rapid methods allowing enumeration of lactic acid bacteria producing biogenic amines in wines and to analyse wine samples by the methods. Methods and Results: Methods based on quantitative PCR targeting bacterial genes involved in histamine, tyramine and putrescine production were developed and applied to detect and quantify the bacteria producing these biogenic amines in wine. Analysis of 102 samples revealed low populations of the targeted bacteria in grape must samples, an increased bacteria biomass in wine samples after alcoholic fermentation, reaching the highest population levels (above 106 cells ml−1) during spontaneous malolactic fermentation. A minimum of 103 ml−1 producing cells was required for production of more than 1 mg l−1 of biogenic amines. Accumulation of putrescine in wine was correlated with the presence of bacteria carrying an ornithine decarboxylation pathway. Trials of winemaking showed that the use of selected bacteria for inducing malolactic fermentation was efficient to limit the proliferation of undesirable bacteria and the production of biogenic amines. Conclusion: Methods using quantitative PCR are efficient to enumerate biogenic amines-producing cells in wine. Significance and Impact of the Study: The methods can help to better control and to improve winemaking conditions in order to avoid biogenic amine production.
Fichier non déposé

Dates et versions

hal-02668490 , version 1 (31-05-2020)

Identifiants

Citer

F. Nannelli, Olivier Claisse, Emmanuel Gindreau, Gilles de Revel, Aline Lonvaud-Funel, et al.. Determination of lactic acid bacteria producing biogenic amines in wine by quantitative PCR methods. Letters in Applied Microbiology, 2008, 47 (6), pp.594 - 599. ⟨10.1111/j.1472-765X.2008.02472.x⟩. ⟨hal-02668490⟩

Collections

INRA INRAE
10 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More