Gene transcription in Lactarius quietus-Quercus petraea ectomycorrhizas from a forest soil - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2008

Gene transcription in Lactarius quietus-Quercus petraea ectomycorrhizas from a forest soil

Résumé

Extracting fungal mRNA from ectomycorrhizas (ECMs) and forest soil samples for monitoring in situ metabolic activities is a significant challenge when studying the role of ECMs in biogeochemical cycles. A robust, simple, rapid, and effective method was developed for extracting RNA from rhizospheric soil and ECMs by adapting previous grinding and lysis methods. The quality and yield of the extracted RNA were sufficient to be used for reverse transcription. RNA extracted from ECMs of Lactarius quietus in a 100-year-old oak stand was used to construct a cDNA library and sequence expressed sequence tags. The transcripts of many genes involved in primary metabolism and in the degradation of organic matter were found. The transcription levels of four targeted fungal genes (glutamine synthase, a general amino acid transporter, a tyrosinase, and N-acetylhexosaminidase) were measured by quantitative reverse transcription-PCR in ECMs and in the ectomycorrhizospheric soil (the soil surrounding the ECMs containing the extraradical mycelium) in forest samples. On average, levels of gene expression for the L. quietus ECM root tips were similar to those for the extraradical mycelium, although gene expression varied up to 10-fold among the samples. This study demonstrates that gene expression from ECMs and soil can be analyzed. These results provide new perspectives for investigating the role of ectomycorrhizal fungi in the functioning of forest ecosystems.
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Dates et versions

hal-02659848 , version 1 (30-05-2020)

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Pierre-Emmanuel Courty, M Poletto, F. Duchaussoy, Marc Buée, Jean J. Garbaye, et al.. Gene transcription in Lactarius quietus-Quercus petraea ectomycorrhizas from a forest soil. Applied and Environmental Microbiology, 2008, 74 (21), pp.6598-6605. ⟨10.1128/AEM.00584-08⟩. ⟨hal-02659848⟩
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