Gene transfer system derived from the caprine arthritis-encephalitis lentivirus - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Virological Methods Année : 2006

Gene transfer system derived from the caprine arthritis-encephalitis lentivirus

Résumé

Lentiviruses are attractive candidates for therapeutic vectors, because of their ability to infect non-dividing target cells. Vectors based on HIV-1 efficiently transfer gene expression to a variety of dividing or quiescent cells, but are subject to reservations on safety grounds. Caprine arthritis encephalitis virus (CAEV) is a lentivirus inducing only minor pathology in its natural host and in related species after cross-species transmission. To test the CAEV potential as vector for gene transfer, a cassette expressing the green fluorescent protein (GFP) under control of a CMV promoter was inserted into the CAEV genome, producing the pK2EGFPH vector. When pseudotyped with vesicular stomatitis virus (VSV)-G envelope protein, this vector allowed efficient transfer of GFP expression in human cells (up to 86% of GFP-expressing cells into the TE671 cell line). Three vectors carrying different parts of the viral gag, pol and env genes were then developed, together with a CAEV packaging system. These vectors allowed delimitation of the minimal CAEV sequences necessary for an improvement of vector production compared to the previously described CAEV-based vectors [Mselli-Lakhal et al., 1998. Defect in RNA transport and packaging are responsible for low transduction efficiency of CAEV-based vectors. Arc. Virol. 143, 681-695]. While our previous vectors were produced in a helper/vector system, the present vectors are produced in a helper/free system. However, these vector titers remain lower than those obtained with other lentiviral vectors carrying equivalent packaging sequences. We discuss on possible reasons of such differences and possible improvements.

Mots clés

Domaines

Virologie

Dates et versions

hal-02655564 , version 1 (29-05-2020)

Identifiants

Citer

Laila Mselli-Lakhal, François Guiguen, Timothy Greenland, Jean-François Mornex, Yahia Chebloune. Gene transfer system derived from the caprine arthritis-encephalitis lentivirus. Journal of Virological Methods, 2006, 136 (1-2), pp.177-184. ⟨10.1016/j.jviromet.2006.05.006⟩. ⟨hal-02655564⟩
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More