Role of Glycoside Phosphorylases in Mannose Foraging by Human Gut Bacteria - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2013

Role of Glycoside Phosphorylases in Mannose Foraging by Human Gut Bacteria

Résumé

To metabolize both dietary fiber constituent carbohydrates and host glycans lining the intestinal epithelium, gut bacteria produce a wide range of carbohydrate-active enzymes, of which glycoside hydrolases are the main components. In this study, we describe the ability of phosphorylases to participate in the breakdown of human N-glycans, from an analysis of the substrate specificity of UhgbMP, a mannoside phosphorylase of the GH130 protein family discovered by functional metagenomics. UhgbMP is found to phosphorolyze -d-Manp-1,4--d-GlcpNAc-1,4-d-GlcpNAc and is also a highly efficient enzyme to catalyze the synthesis of this precious N-glycan core oligosaccharide by reverse phosphorolysis. Analysis of sequence conservation within family GH130, mapped on a three-dimensional model of UhgbMP and supported by site-directed mutagenesis results, revealed two GH130 subfamilies and allowed the identification of key residues responsible for catalysis and substrate specificity. The analysis of the genomic context of 65 known GH130 sequences belonging to human gut bacteria indicates that the enzymes of the GH130_1 subfamily would be involved in mannan catabolism, whereas the enzymes belonging to the GH130_2 subfamily would rather work in synergy with glycoside hydrolases of the GH92 and GH18 families in the breakdown of N-glycans. The use of GH130 inhibitors as therapeutic agents or functional foods could thus be considered as an innovative strategy to inhibit N-glycan degradation, with the ultimate goal of protecting, or restoring, the epithelial barrier.
Fichier principal
Vignette du fichier
2013_Ladevèze_Journal of Biological Chemistry_1.pdf (2.66 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02650371 , version 1 (29-05-2020)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Simon Ladeveze, Laurence Tarquis, Davide Cecchini, Juliette Bercovici, Isabelle André, et al.. Role of Glycoside Phosphorylases in Mannose Foraging by Human Gut Bacteria. Journal of Biological Chemistry, 2013, 288 (45), pp.32370-32383. ⟨10.1074/jbc.M113.483628⟩. ⟨hal-02650371⟩
13 Consultations
28 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More