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Article Dans Une Revue Molecular Microbiology Année : 2016

Escherichia coliClbS is a colibactin resistance protein

Résumé

The genomic pks island codes for the biosynthetic machinery that produces colibactin, a peptide-polyketide metabolite. Colibactin is a genotoxin that contributes to the virulence of extra-intestinal pathogenic Escherichia coli and promotes colorectal cancer. In this work, we examined whether the pks-encoded clbS gene of unknown function could participate in the self-protection of E. coli-producing colibactin. A clbS mutant was not impaired in the ability to inflict DNA damage in HeLa cells, but the bacteria activated the SOS response and ceased to replicate. This autotoxicity phenotype was markedly enhanced in a clbSuvrB double mutant inactivated for DNA repair by nucleotide excision but was suppressed in a clbSclbA double mutant unable to produce colibactin. In addition, ectopic expression of clbS protected infected HeLa cells from colibactin. Thus, ClbS is a resistance protein blocking the genotoxicity of colibactin both in the procaryotic and the eucaryotic cells.

Dates et versions

hal-02640348 , version 1 (28-05-2020)

Identifiants

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Nadège Bossuet-Greif, Damien Dubois, Claude Petit, Sophie Tronnet, Patricia Martin, et al.. Escherichia coliClbS is a colibactin resistance protein. Molecular Microbiology, 2016, 99 (5), pp.897-908. ⟨10.1111/mmi.13272⟩. ⟨hal-02640348⟩
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