Thulium spiked gel for internal standardisation in LA-ICP-MS bio-imaging: quantitative elemental distribution of uranium in kidney tissue - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2019

Thulium spiked gel for internal standardisation in LA-ICP-MS bio-imaging: quantitative elemental distribution of uranium in kidney tissue

Résumé

The quantitative analysis of trace metals in different organs or cellular structures is a topic of emerging interest for the assessment of toxicological risk. The kidney is recognized as a major site for uranium accumulation able to induce renal toxicity. Due to its heterogeneous distribution within the tissue, the quantification appears to be of significant interest. Mass spectrometry imaging (MSI) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been employed for mapping and quantifying uranium in histological tissue sections of mouse kidney. Samples were immediately immersed into a PFA solution during 24h, OCT embedded and stored at -80°C until cutting in cryostat and mounting in gel covered glass slides. Sample preparation (tissue section thickness) and laser conditions were carefully optimized to assure the complete ablation of the sample matrix. This works presents the development of internal standard correction methodology based on doped gelatine. In order to correct matrix effects, lack of tissue homogeneity and instrumental drift, a thulium (Tm, internal standard) spiked 10% (m/v) gel was prepared and deposited on the top of glass microscope slides. The reproducibility and homogeneity of the deposited gel was assessed by ablating several areas (0.5 x 0.5 mm, n=20) in which Tm was recorded. For quantification purposes, matrix-matched laboratory standards were prepared from a pool of mouse kidneys by dosing each level with different concentration of uranium. In the same way, several quality control (QC) samples were synthetized to prove the accuracy and precision of the analytical method. The unspiked tissue was used for blank control. The concentrations were verified by conventional liquid ICP-MS after acid digestion. These standards were cut and mounted onto glass slides in the same way as the samples. Thulium spiked gel was demonstrated to be adequate for compensation of instrumental drifts during measuring time and matrix effects, which improves the quantification of elemental distributions and allows a trustworthy comparison of experiments performed at different times. The proposed analytical bio-imaging approach was successfully applied for quantification of uranium of kidney samples. The comparison of the calculated average concentration obtained by LA-ICP-MS and the results obtained after liquid ICP-MS analysis were in good accordance.
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hal-02635599 , version 1 (27-05-2020)

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  • HAL Id : hal-02635599 , version 1

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Nagore Grijalba Marijuan, Alexandre Legrand, Yann Gueguen, Valerie Holler, Celine Bouvier Capely. Thulium spiked gel for internal standardisation in LA-ICP-MS bio-imaging: quantitative elemental distribution of uranium in kidney tissue. European Winter Conference on plasma Spectrochemistry, EWCPS 2019, Feb 2019, PAU, France. 2019. ⟨hal-02635599⟩
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