<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02632926</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-14T11:52:01+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">The &lt;em&gt;Medicago truncatula MtRbohE&lt;/em&gt; gene is activated in arbusculated cells and is involved in root cortex colonization</title>
            <author role="aut">
              <persName>
                <forename type="first">Simone</forename>
                <surname>Belmondo</surname>
              </persName>
              <idno type="halauthorid">1796797-0</idno>
              <affiliation ref="#struct-576679"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Cristina</forename>
                <surname>Calcagno</surname>
              </persName>
              <idno type="halauthorid">1880439-0</idno>
              <affiliation ref="#struct-576679"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Andrea</forename>
                <surname>Genre</surname>
              </persName>
              <idno type="halauthorid">1235731-0</idno>
              <affiliation ref="#struct-576679"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alain</forename>
                <surname>Puppo</surname>
              </persName>
              <idno type="halauthorid">1052019-0</idno>
              <affiliation ref="#struct-199347"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nicolas</forename>
                <surname>Pauly</surname>
              </persName>
              <email type="md5">6a9125c4b6b28b288127abf3b1bb1dd4</email>
              <email type="domain">inrae.fr</email>
              <idno type="idhal" notation="string">nicolas-pauly</idno>
              <idno type="idhal" notation="numeric">735820</idno>
              <idno type="halauthorid" notation="string">36798-735820</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-2990-3150</idno>
              <idno type="IDREF">https://www.idref.fr/249963302</idno>
              <idno type="VIAF">https://viaf.org/viaf/713160483685604990951</idno>
              <affiliation ref="#struct-199347"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Luisa</forename>
                <surname>Lanfranco</surname>
              </persName>
              <idno type="halauthorid">1243799-0</idno>
              <affiliation ref="#struct-576679"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Nicolas</forename>
                <surname>PAULY</surname>
              </persName>
              <email type="md5">8176b9174665525ff35cdb6b5f72da5b</email>
              <email type="domain">ensat.fr</email>
            </editor>
            <funder>BIOBIT-Converging Technology project (WP2); ANR [BLAN07-2_182872]</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2024-09-09 07:49:13</date>
              <date type="whenModified">2025-06-24 03:41:57</date>
              <date type="whenReleased">2024-09-09 07:51:14</date>
              <date type="whenProduced">2016</date>
              <date type="whenEndEmbargoed">2024-09-09</date>
              <ref type="file" target="https://hal.inrae.fr/hal-02632926v1/document">
                <date notBefore="2024-09-09"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.inrae.fr/hal-02632926v1/file/Belmondo%2Bet%2Bal%2B2016%2BPlanta.pdf" id="file-4691600-4078853">
                <date notBefore="2024-09-09"/>
              </ref>
              <ref type="externalLink" target="https://iris.unito.it/bitstream/2318/1535191/12/Belmondo%20et%20al%202016%20Planta.pdf"/>
              <fs>
                <f name="inra_publicVise_local" notation="string" n="SC">
                  <string>inra_publicVise_local_SC</string>
                </f>
              </fs>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="878849">
                <persName>
                  <forename>Nicolas</forename>
                  <surname>PAULY</surname>
                </persName>
                <email type="md5">8176b9174665525ff35cdb6b5f72da5b</email>
                <email type="domain">ensat.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02632926</idno>
            <idno type="halUri">https://hal.inrae.fr/hal-02632926</idno>
            <idno type="halBibtex">belmondo:hal-02632926</idno>
            <idno type="halRefHtml">&lt;i&gt;Planta&lt;/i&gt;, 2016, 243 (1), pp.251-262. &lt;a target="_blank" href="https://dx.doi.org/10.1007/s00425-015-2407-0"&gt;&amp;#x27E8;10.1007/s00425-015-2407-0&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Planta, 2016, 243 (1), pp.251-262. &amp;#x27E8;10.1007/s00425-015-2407-0&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-4691600-4078853"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="SDE">Sciences De l'Environnement</idno>
            <idno type="stamp" n="UNICE">Université Nice Sophia Antipolis</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="GIP-BE">GIP Bretagne Environnement</idno>
            <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
            <idno type="stamp" n="UNIV-COTEDAZUR">Université Côte d'Azur</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
            <idno type="stamp" n="INRAEPACA">INRAE PACA</idno>
            <idno type="stamp" n="ISA-SOPHIA">Institut Sophia Agrobiotech</idno>
            <idno type="stamp" n="UNIV-COTEDAZUR_COLLECTION_DEFAUT">UNIV-COTEDAZUR_COLLECTION_DEFAUT</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">The &lt;em&gt;Medicago truncatula MtRbohE&lt;/em&gt; gene is activated in arbusculated cells and is involved in root cortex colonization</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Simone</forename>
                    <surname>Belmondo</surname>
                  </persName>
                  <idno type="halauthorid">1796797-0</idno>
                  <affiliation ref="#struct-576679"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Cristina</forename>
                    <surname>Calcagno</surname>
                  </persName>
                  <idno type="halauthorid">1880439-0</idno>
                  <affiliation ref="#struct-576679"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Andrea</forename>
                    <surname>Genre</surname>
                  </persName>
                  <idno type="halauthorid">1235731-0</idno>
                  <affiliation ref="#struct-576679"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alain</forename>
                    <surname>Puppo</surname>
                  </persName>
                  <idno type="halauthorid">1052019-0</idno>
                  <affiliation ref="#struct-199347"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nicolas</forename>
                    <surname>Pauly</surname>
                  </persName>
                  <email type="md5">6a9125c4b6b28b288127abf3b1bb1dd4</email>
                  <email type="domain">inrae.fr</email>
                  <idno type="idhal" notation="string">nicolas-pauly</idno>
                  <idno type="idhal" notation="numeric">735820</idno>
                  <idno type="halauthorid" notation="string">36798-735820</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-2990-3150</idno>
                  <idno type="IDREF">https://www.idref.fr/249963302</idno>
                  <idno type="VIAF">https://viaf.org/viaf/713160483685604990951</idno>
                  <affiliation ref="#struct-199347"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Luisa</forename>
                    <surname>Lanfranco</surname>
                  </persName>
                  <idno type="halauthorid">1243799-0</idno>
                  <affiliation ref="#struct-576679"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">7828</idno>
                <idno type="issn">0032-0935</idno>
                <idno type="eissn">1432-2048</idno>
                <title level="j">Planta</title>
                <imprint>
                  <publisher>Springer Verlag</publisher>
                  <biblScope unit="volume">243</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">251-262</biblScope>
                  <date type="datePub">2016</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/s00425-015-2407-0</idno>
              <idno type="prodinra">349707</idno>
              <idno type="pubmed">26403286</idno>
              <idno type="wos">000367614800017</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Respiratory burst oxidase homolog</term>
                <term xml:lang="en">Reactive oxygen species</term>
                <term xml:lang="en">NADPH oxidase</term>
                <term xml:lang="en">Medicago</term>
                <term xml:lang="en">Gene expression</term>
                <term xml:lang="en">Arbuscular mycorrhizal symbiosis</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halDomain" n="sde">Environmental Sciences</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Our study demonstrated that the NAPDH oxidase gene MtRbohE is expressed in arbusculated cells and plays a role in arbuscule development. Plant NADPH oxidases, known as respiratory burst oxidase homologs (RBOH), belong to a multigenic family that plays an important role in the regulation of plant development and responses to biotic and abiotic stresses. In this study, we monitored the expression profiles of five Rboh genes (MtRbohA, MtRbohB, MtRbohE, MtRbohG, MtRbohF) in the roots of the model species Medicago truncatula upon colonization by arbuscular mycorrhizal fungi. A complementary cellular and molecular approach was used to monitor changes in mRNA abundance and localize transcripts in different cell types from mycorrhizal roots. Rboh transcript levels did not drastically change in total RNA extractions from whole mycorrhizal and non-mycorrhizal roots. Nevertheless, the analysis of laser microdissected cells and Agrobacterium rhizogenes-transformed roots expressing a GUS transcriptional fusion construct highlighted the MtRbohE expression in arbuscule-containing cells. Furthermore, the down regulation of MtRbohE by an RNAi approach generated an altered colonization pattern in the root cortex, when compared to control roots, with fewer arbuscules and multiple penetration attempts. Altogether our data indicate a transient up-regulation of MtRbohE expression in cortical cells colonized by arbuscules and suggest a role for MtRbohE in arbuscule accommodation within cortical cells.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-576679" status="INCOMING">
          <orgName>Universita di Torino</orgName>
          <desc>
            <address>
              <country key="IT"/>
            </address>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-199347" status="OLD">
          <orgName>Institut Sophia Agrobiotech</orgName>
          <orgName type="acronym">ISA</orgName>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>INRA Centre de recherche Provence-Alpes-Côte d'Azur 400, route des Chappes BP 167 06903 Sophia Antipolis Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.paca.inra.fr/institut-sophia-agrobiotech</ref>
          </desc>
          <listRelation>
            <relation name="UMR1355" active="#struct-92114" type="direct"/>
            <relation active="#struct-117617" type="direct"/>
            <relation name="UMR7254" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-92114" status="OLD">
          <idno type="ROR">https://ror.org/01x3gbx83</idno>
          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-117617" status="VALID">
          <idno type="IdRef">026403498</idno>
          <idno type="ISNI">0000000123372892</idno>
          <idno type="ROR">https://ror.org/02k9vew78</idno>
          <orgName>Université Nice Sophia Antipolis (1965 - 2019)</orgName>
          <orgName type="acronym">UNS</orgName>
          <date type="start">1965-10-23</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Parc Valrose, 06100 Nice</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://unice.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>