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Article Dans Une Revue (Article De Synthèse) Molecular Aspects of Medicine Année : 2018

Laser microdissection: A powerful tool for genomics at cell level

Résumé

Laser microdissection (LM) has become widely democratized over the last fifteen years. Instruments have evolved to offer more powerful and efficient lasers as well as new options for sample collection and preparation. Technological evolutions have also focused on the post-microdissection analysis capabilities, opening up investigations in all disciplines of experimental and clinical biology, thanks to the advent of new high-throughput methods of genome analysis, including RNAseq and proteomics, now globally known as microgenomics, i.e. analysis of biomolecules at the cell level. In spite of the advances these rapidly developing methods have allowed, the workflow for sampling and collection by LM remains a critical step in insuring sample integrity in terms of histology (accurate cell identification) and biochemistry (reliable analyzes of biomolecules). In this review, we describe the sample processing as well as the strengths and limiting factors of LM applied to the specific selection of one or more cells of interest from a heterogeneous tissue. We will see how the latest developments in protocols and methods have made LM a powerful and sometimes essential tool for genomic and proteomic analyzes of tiny amounts of biomolecules extracted from few cells isolated from a complex tissue, in their physiological context, thus offering new opportunities for understanding fundamental physiological and/or pathophysiological processes.
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Dates et versions

hal-02621142 , version 1 (26-05-2020)

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Claudia Bevilacqua, Bertrand Ducos. Laser microdissection: A powerful tool for genomics at cell level. Molecular Aspects of Medicine, 2018, 59, pp.5-27. ⟨10.1016/j.mam.2017.09.003⟩. ⟨hal-02621142⟩
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