Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2010

Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences

Résumé

A common problem in research laboratories that study the mammalian embryo is the limited supply of live material. For this reason, new methods are constantly being developed and existing methods for in vitro models using cells in culture are being adapted to represent embryogenesis. Three-dimensional fluorescence in situ hybridization (3D-FISH) is an important tool to study where genomic sequences are positioned within nuclei without interfering with this 3D organization. When used in the embryo, this technique provides vital information about the distribution of specific sequences in relation to embryonic nuclear substructures such as nucleolar precursor bodies and chromocenters. In this chapter, we will present a detailed description of FISH in order to perform 3D-FISH in the early preimplantation murine embryos.
Fichier principal
Vignette du fichier
3D_FISH_MethMolBiol_Maalouf_Beaujean.pdf (564 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02610638 , version 1 (17-05-2020)

Identifiants

Citer

Walid W. Maalouf, Tiphaine Aguirre-Lavin, Laetitia Herzog, Isabelle Bataillon, Pascale Debey, et al.. Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences. Fluorescence in situ Hybridization (FISH), 659 (4), Springer, pp.401-408, 2010, Methods in Molecular Biology, ⟨10.1007/978-1-60761-789-1_31⟩. ⟨hal-02610638⟩
19 Consultations
266 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More