Combining fieldwork and surface data, we have reconstructed the Cenozoic structural and tectonic evolution of the Northern Bresse. Analysis of drainage network geometry allowed to detect three major fault zones trending NE-SW, E-W and NW-SE, and smooth folds with NNE trending axes, all corroborated with shallow well data in the graben and fieldwork on edges. Cenozoic paleostress succession was determined through fault slip and calcite twin inversions, taking into account data of relative chronology. A N-S major compression, attributed to the Pyrenean orogenesis, has activated strike-slip faults trending NNE along the western edge and NE-SW in the graben. After a transitional minor E-W trending extension, the Oligocene WNW extension has structured the graben by a collapse along NNE to NE-SW normal faults. A local NNW extension closes this phase. The Alpine collision has led to an ENE compression at Early Miocene. The following WNW trending major compression has generated shallow deformation in Bresse, but no deformation along the western edge. The calculation of potential reactivation of pre-existing faults enables to propose a structural sketch map for this event, with a NE-SW trending transfer fault zone, inactivity of the NNE edge faults, and possibly large wavelength folding, which could explain the deposit agency and repartition of Miocene to Quaternary deformation.