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Article Dans Une Revue Journal of Photochemistry and Photobiology A: Chemistry Année : 2020

Nitroreductase sensitive styryl-benzothiazole profluorescent probes for the visualization of mitochondria under normoxic conditions

Résumé

Selective imaging of cellular organelles is a rapidly expanding field of research. Within this context we describe the development of two novel profluorescent probes, MitoP-2 and MitoP-3, for the precise staining of mitochondrial compartments. Herein, we demonstrate that caged, non-fluorescent styryl-benzothiazole probes harbouring a p-nitrobenzyl motif can be converted into their parent fluorophore under the action of the nitroreductase (NTR) enzyme. Two probes, MitoP-2 and MitoP-3, exhibited a turn-ON fluorescence signalling in NTR enzymatic assays. In addition, they also proved successful in the staining of the mitochondria in cellular experiments under normoxic conditions. Interestingly, while MitoP-2 and MitoP-3 structures only differ by the presence of a cationic mitochondrial-targeting unit, both were equally efficient in the staining of mitochondria. Hence, an activity-based approach (MitoP-2) is sufficient for the precise targeting of mitochondria and the use of cationic mitochondria-targeting units (phosphonium present on MitoP-3) can be considered superfluous in this case.
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Dates et versions

hal-02567124 , version 1 (07-05-2020)

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Mauro Safir Filho, Pascal Dao, Anthony Martin, Rachid Benhida. Nitroreductase sensitive styryl-benzothiazole profluorescent probes for the visualization of mitochondria under normoxic conditions. Journal of Photochemistry and Photobiology A: Chemistry, inPress, 396, pp.112528. ⟨10.1016/j.jphotochem.2020.112528⟩. ⟨hal-02567124⟩
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